Plantlet Regeneration from Meristems of Philippine Sweetpotato Varieties


Author: Villaluz Z. Acedo

Abstract

The solidified Murashige and Skoog (MS) medium added with gibberellic acid (GA3) induced direct organogenesis without any callus formation of the meristems of sweetpotato, varieties VSP-1, VSP-2 and VSP-3. The optimum GA3 concentration varied with variety. On the other hand, benzylaminopurine as medium supplement caused profuse callus growth on the basal portions of the meristem before shoots developed.
Shoot growth of the meristems was much faster in liquid than in solid MS medium supplemented with GA3. Rapid shoot and root development was induced when the cultures were transferred to the solid MS medium containing high sucrose level 1.5 month after.

Keywords : Sweetpotato. Ipomoea batatas L. Meristem culture. Organogenesis. Murashige and Skoog. BAP. GA3 .

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