Incidence of sweetpotato viruses in Central Luzon, Philippines


Author(s): Erlinda A. Vasquez1, Manuel K. Palomar2, Lilibeth B. Laranang3 and Edgardo B. Barsalote1

Abstract

Sweetpotao commercial growing areas in Central Luzon particularly Tarlac and Bataan were surveyed for the incidence of sweetpotato viruses. Healthy-looking sweetpotato leaves and those showing symptoms of virus infection were collected and subjected to nitrocellulose membrane-enzyme linked immunoabsorbent serological assay (NCM-ELISA) for the detection of known viruses using the kit and protocol from Centro Internacional de la Papa, Lima Peru. The NCM-ELISA detected sweetpotato feathery mottle virus (SPFMV), sweetpotato mild mottle virus (SPMMV), sweetpotato latent virus (SPLV), sweetpotao chlorotic fleck virus (SPCFV), C-6 virus, sweetpotato mild speckling virus (SPMSV), sweetpotato caulimo-like virus (SPCalV), and sweetpotato chlorotic stunt virus (SPCSV). Six to 8 viruses were found in Tarlac and 3-4 viruses in Bataan. Highest infections were recorded for C-6, SPMSV, SPCalV and SPCSV reaching 100% in almost all areas. Infection of SPFMV ranged from 45-70%. The combination of SPFMV with other viruses was common registering 40-70% complex infection. Mixed infection of 3-6 viruses (SPFMV, SPMMV, SPLV, C-6, SPMSV and SPCalV) was found in Tarlac and 2-4 viruses (SPFMV, C-6, SPMSV and SPCalV) in Bataan. Practically all sweetpotato growing areas of Tarlac are “hotspot” areas of sweetpotato viruses.

Keywords : sweetpotato virus diseases, identification, incidence, SPFMV, SPMMV,SPLV, SPCFV, C-6, SPMSV, SPCalV, SPCSV, serological test, NCM-ELISA, Central Luzon

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